Fig 3. Efficiency of CRISPR/Cas editing of Bos1 using repair templates with short homology flanks.

(A) Experimental scheme. (B) Results of transformations with RNP (black lines) and without RNP (blue lines) for RT with flank sizes of 0 to 60 bp. (+RNP: n = 4; -RNP: n = 3). The numbers below show fractions of Fen^R transformants being Ipr^R, indicating targeting efficiencies. The p values by one-way ANOVA followed by Dunnett’s multiple comparisons (control: 0 bp) post hoc test are indicated. ***p ≤ 0.001. (C) Scheme of Bos1 targeting with different placement of 60 bp homology flanks of RT, resulting in insertion (left) or 2 kb deletion (right). (D) Results of transformations with RNP and two types of RT as shown in (C). n = 5 (Ipr^R); n = 4 (Fen^R). The p values by one-way ANOVA followed by Tukey’s multiple comparisons post hoc test are indicated. *p ≤ 0.05; ** p ≤ 0,01.