Fig 4. Application of marker exchange for non-selected CRISPR/Cas mutagenesis of Bos1 via NHEJ.
(A) Experimental scheme. (B) Results of transformation of B. cinerea xyn11A-NatR with Cas9-RNP targeting nat using FenR repair template, with or without Cas9-RNP targeting Bos1, as shown in (B). Control: FenR repair template added alone.