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. 2020 Aug 17;16(8):e1008326. doi: 10.1371/journal.ppat.1008326

Fig 4. Application of marker exchange for non-selected CRISPR/Cas mutagenesis of Bos1 via NHEJ.

Fig 4

(A) Experimental scheme. (B) Results of transformation of B. cinerea xyn11A-NatR with Cas9-RNP targeting nat using FenR repair template, with or without Cas9-RNP targeting Bos1, as shown in (B). Control: FenR repair template added alone.