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. 2020 Aug 17;16(8):e1008326. doi: 10.1371/journal.ppat.1008326

Fig 6. Efficient pTEL-mediated editing via NHEJ or HR in Magnaporthe oryzae.

Fig 6

(A) and (B) Guy11 protoplasts were cotransformed with pTEL-Fen and Cas9-MoALB1-sgRNA RNP. (A) Primary selection plate containing Fen. (B) Isolated transformants (cf. S2 Table). Note white-colored mycelia of edited transformants. (C) and (D) Guy11 and Guy11ku80 protoplasts were cotransformed with pTEL-Fen, Cas9-MoPIT-sgRNA RNP and HygR-MoPIT RT. (C) Fraction of FenR transformants which were also HygR. (D) Editing rate, defined as the fraction of FenR transformants with correct edits via HR. Values of Guy11 and Guy11ku80 were compared by unpaired t-test. *p ≤ 0.05 (n = 4).