Table 3. Cofitness scores of select metabolic modules.
| Module No. | Average Cofitnessa | No. of Rxn | Coupled Reactionsb | No. of Genes | Relevant Mutantsc | Associated Pathways |
|---|---|---|---|---|---|---|
| Modules with the highest average cofitness scores | ||||||
| M1 | 0.880 | 4 | ALAS_f, HMBS_f, PPBNGS_f, UPP3S_f | 4 | ZMO1198, ZMO1879, ZMO1903 | Porphyrinogen Biosynthesis |
| M2 | 0.859 | 3 | CHORS_f, PSCVT_f, SHKK_f | 3 | ZMO0594, ZMO1693, ZMO1796 | Chorismate biosynthesis |
| M3 | 0.846 | 4 | ASPCT_f, DHORTS_r, OMPDC_f, ORPT_r | 3 | ZMO0587, ZMO0791, ZMO1707 | Uridine biosynthesis |
| Modules associated with histidine and cofactor biosynthesis | ||||||
| M10 | 0.746 | 10 | ATPPRT_f, HISTD_f, HISTP_f, HSTPT_f, IG3PS_f, IGPDH_f, PRAMPC_f, PRATPP_f, PRMICI_f, SINK_his-L_f | 9 | ZMO0421, ZMO1178, ZMO1499, ZMO1500, ZMO1501, ZMO1502, ZMO1503, ZMO1550, ZMO1551 | Histidine biosynthesis |
| M46 | 0.470 | 19 | AMAOTr_f, AOXSr2_f, BTS5_f, DBTS_f, DM_AMOB_f, EGMEACPR_f, EPMEACPR_f, EX_meoh_e_f, MALCOAMT_f, MEOHtex_r, MEOHtrpp_r, OGMEACPD_f, OGMEACPR_f, OGMEACPS_f, OPMEACPD_f, OPMEACPR_f, OPMEACPS_f, PMEACPE_f, S2FE2SR_f | 13 | ZMO0094, ZMO0423, ZMO0425, ZMO0426, ZMO0427, ZMO1067, ZMO1146, ZMO1222, ZMO1278, ZMO1692, ZMO1915, ZMO1917, ZMO1918, | Biotin biosynthesis |
| Modules with average cofitness scores below the significance threshold | ||||||
| M45 | 0.497 | 5 | E4PD_f, OHPBAT_f, PDX5PS_f, PERD_f, SINK_pydx5p_f | 3 | ZMO1313, ZMO1684, ZMO1708 | Pyridoxine biosynthesis |
| M51 | 0.346 | 8 | EX_h2_e`_f, EX_n2_e_r, FNOR_r, H2tex_r, H2tpp_r, N2tex_f, N2tpp_f, NIT1b_f | 3 | ZMO1823, ZMO1824, ZMO1825 | Nitrogen fixation |
| M52 | 0.332 | 5 | CDPMEK_f, DXPRIi_f, MECDPDH5_f, MECDPS_f, MEPCT_f | 4 | ZMO0180, ZMO1128, ZMO1182, ZMO1851 | Isoprenoid Precursor biosynthesis |
| M56 | 0.293 | 3 | PGCD_f, PSERT_f, PSP_L_f | 3 | ZMO1137, ZMO1684, ZMO1685 | Serine biosynthesis |
| M57 | 0.247 | 6 | ADEt2rpp_r, ADEtex_r, HPN1_f, HPN2_f, HPN3_f, EX_ade_e_f | 3 | ZMO0873, ZMO0874, ZMO0969 | Hopanoid biosynthesis |
| M60 | 0.119 | 11 | ADCL_f, ADCS_f, AKP1_f, DHFS_f, DHNPA2r_f, DHPS2_f, EX_gcald_e_f, GCALDtex_f, GCALDtpp_f, GTPCI_f, HPPK2_f | 7 | ZMO0113, ZMO0114, ZMO0582, ZMO0938, ZMO1006, ZMO1229, ZMO1277 | Folate biosynthesis |
| M63 | -0.140 | 7 | AMPMS2_f, DM_4CRSOL_f, ICYSDS_f, PMPK_f, THZPSN3_f, TMPPP_f, TYRL_f | 3 | ZMO0172, ZMO0738, ZMO1834 | Thiamine biosynthesis |
a Module number shows relative position of module based on sorted average cofitness values.
b Suffix "_f" represents forward component and "_r" represents reverse component of the reaction. Reactions italicized either had no available mutant in the collection, or had isozymes confounding analysis. Underlined reactions represent exchange or sink reactions missing GPRs. Bolded reactions represent reactions missing GPRs.
c Mutants whose genes are associated with the reactions in the module excluding known isozymes and mutants that are absent from the Tn5 mutant collection.