Table 2.

Thermocycler variables associated with long- and short-mitochondrial primers.

Phase	Long Mito Primers	Short Mito Primers
Melting	94 °C for 4 min	94 °C for 1 min
Amplification	26-28 cycles of melting (94 °C for 15 s)	20 cycles of melting (94 °C for 15 s)
Annealing	66 °C for 12 min	60 °C for 45 s
Final Extension	72 °C for 10 min	72 °C for 45 s
Hold	4 °C (or 8 °C if being held overnight)	4 °C (or 8 °C if being held overnight)