Table 2.
Phase | Long Mito Primers | Short Mito Primers |
---|---|---|
Melting | 94 °C for 4 min | 94 °C for 1 min |
Amplification | 26-28 cycles of melting (94 °C for 15 s) | 20 cycles of melting (94 °C for 15 s) |
Annealing | 66 °C for 12 min | 60 °C for 45 s |
Final Extension | 72 °C for 10 min | 72 °C for 45 s |
Hold | 4 °C (or 8 °C if being held overnight) | 4 °C (or 8 °C if being held overnight) |