Table 1.
Effects of RVV and its fractions (RvPLA2, RvMP, RvLAAO, and RvPDE) with or without WEB 2086 pre-treatment on the urinary NO concentration in the rabbit IPK.
| Variables: groups | Basal | 5 min | 10 min | 30 min | 60 min | 90 min |
|---|---|---|---|---|---|---|
| Urinary NO (mM): | ||||||
| Control | 0.75 ± 0.02 | 0.74 ± 0.04 | 0.75 ± 0.04 | 0.78 ± 0.06 | 0.80 ± 0.10 | 0.78 ± 0.02 |
| WEB 2086 | 0.88 ± 0.16 | 0.77 ± 0.11 | 0.79 ± 0.10 | 0.79 ± 0.06 | 0.83 ± 0.12 | 0.80 ± 0.08 |
| RVV | 1.05 ± 0.06 | 0.98 ± 0.18 | 1.03 ± 0.16 | 0.99 ± 0.10 | 0.97 ± 0.06 | 1.08 ± 0.06 |
| WEB 2086 + RVV | 0.80 ± 0.08 | 0.82 ± 0.06 | 0.84 ± 0.14 | 0.86 ± 0.12 | 0.88 ± 0.12 | 0.88 ± 0.08 |
| RvPLA2 | 1.26 ± 0.26 | 1.13 ± 0.42 | 1.15 ± 0.18 | 1.08 ± 0.30 | 1.20 ± 0.28 | 1.15 ± 0.24 |
| WEB 2086 + RvPLA2 | 0.84 ± 0.26 | 0.89 ± 0.28 | 0.88 ± 0.34 | 0.85 ± 0.28 | 0.89 ± 0.12 | 0.91 ± 0.14 |
| RvMP | 0.93 ± 0.12 | 0.85 ± 0.42 | 0.88 ± 0.16 | 0.87 ± 0.12 | 0.90 ± 0.06 | 0.97 ± 0.16 |
| WEB 2086 + RvMP | 0.85 ± 0.08 | 1.02 ± 0.06 | 0.90 ± 0.14 | 0.91 ± 0.18 | 0.96 ± 0.10 | 0.87 ± 0.16 |
| RvLAAO | 0.95 ± 0.16 | 0.74 ± 0.18 | 0.94 ± 0.09 | 0.86 ± 0.10 | 0.89 ± 0.24 | 0.88 ± 0.24 |
| WEB 2086 + RvLAAO | 0.91 ± 0.16 | 0.96 ± 0.16 | 0.96 ± 0.04 | 0.87 ± 0.12 | 0.94 ± 0.18 | 1.11 ± 0.12 |
| RvPDE | 0.98 ± 0.26 | 0.97 ± 0.24 | 0.91 ± 0.14 | 0.94 ± 0.16 | 0.89 ± 0.12 | 0.90 ± 0.10 |
| WEB 2086 + RvPDE | 0.86 ± 0.18 | 0.93 ± 0.06 | 0.86 ± 0.18 | 0.94 ± 0.14 | 1.09 ± 0.08 | 0.82 ± 0.10 |
Data are presented as the mean ± SD of four perfused kidneys from four different animals in each group. P-values analyzed by repeated measures ANOVA with Bonferroni post hoc test: *P < 0.05, mean values of specified time period with respect to the basal period in the same group. Basal is the internal control period prior to treatment of each experimental group. WEB 2086 was added 30 min before the specified treatment of each experimental group. Comparison of two sets of data at each specified time point between the treated group alone and the treated group pretreatment with WEB 2086 using unpaired Student's t-test. For calculations, samplings were made at 5, 10, 30, 60, and 90 min after the basal measurements of each experiment. No significant changes in urinary NO concentrations (0.75–1.26 mM) at any point in all treated groups after 90 min of the perfusion time.