Fig. 6.

ME1 and IDH2 synergistically induce cancer cell apoptosis.

A. Effect of IDH2 inhibition by 2.5 μM AGI-6780 for 24 h, ME1 knockdown and the combined treatment on intracellular ROS levels in MCF-7 and A549 cells (n = 3).

B. Effect of IDH2 inhibition by 2.5 μM AGI-6780 for 24 h, ME1 knockdown and the combined treatment on labeled fatty acid abundance levels in MCF-7 and A549 cells cultured in medium containing 10 mM [U-¹³C] glucose for 72 h (n = 4).

C. Effect of IDH2 inhibition by 2.5 μM AGI-6780 for 24 h, ME1 knockdown and the combined treatment on apoptosis rate in MCF-7 and A549 cells (n = 3).

D. Intracellular ROS levels in MCF-7 and A549 cells after ME1 knockdown and dual targeting of ME1 and IDH2 in culture with or without 10 mM NAC treatment for 24 h (n = 3).

E. Relative viability of MCF-7 and A549 cells after ME1 knockdown and dual targeting of ME1 and IDH2 in culture with or without 10 mM NAC treatment for 24 h (n = 5).

F. Effect of combinatorial targeting ME1 and CTP on relative cell viability of MCF-7 and A549 cells (n = 6).

Data are represented as mean ± SD. The experiments in B were conducted once, and all the other experiments were repeated at least twice. Statistical significance was determined by Student's t-test. **p < 0.01, ***p < 0.001. See also Supplementary Fig. 6.