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Osteogenic activity of mBMSCs on various samples in vitro. (a) ALP positive areas, collagen secretion, and extracellular matrix mineralization of mBMSCs cultured on various samples for 10 d in single-culture and co-culture conditions and the corresponding colorimetrically qualitative results. (b) Relative mRNA expression levels of the bone formation-related genes in mBMSCs in single-culture and co-culture conditions on day 10 on sample surfaces.
