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. 2020 Aug 24;13(12):100850. doi: 10.1016/j.tranon.2020.100850

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Relationship between the OPNI and intratumoral CD8+ cell counts in the 47 patients with OSCC.

(A) Box plot showing the number of intratumoral CD8+ cells according to OPNI status. The differences in mean values between the two groups were statistically analyzed using the Mann–Whitney's U test. **, p < .01.

(B) Representative photographs of the results of double-immunohistochemical staining of CD8 (brown) and cytokeratin (green) in OPNI-high tumor. Original magnification: ×400, scale bar = 50 μm.

(C) Representative photographs of the results of double-immunohistochemical staining of CD8 (brown) and cytokeratin (green) in OPNI-low tumor. Original magnification: ×400, scale bar = 50 μm.