Figure 4.

Role of ADAR1 and ADAR2 in Vasoactive MicroRNA Editing and Maturation

(A–E) ADAR1 and ADAR2 were knocked down in HUAFs through transfection of an ADAR1-targeting or ADAR2-targeting siRNA (siADAR1 and siADAR2, respectively). The subsequent effects on microRNA expression and editing were analyzed as in Figures 2 and 3 and compared to transfection with a negative control siRNA (siNegCtrl). (A) pri-MicroRNA A-to-I editing and expression. (B–E) Mature (B) miR-376a&b-3p, (C) miR-376c-3p, (D) miR-381-3p, and (E) miR-411-5p expression relative to U6 (top panels) and the corresponding percentage of mature microRNA-ED (bottom panels). All data are presented as mean ± SEM from three independent experiments performed with pooled cells from 13 different umbilical cords. #p < 0.01, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, versus siNegCtrl or as indicated by a two-sided Student’s t test.