Figure 8.

Functional Effect of WT-MicroRNAs and ED-MicroRNAs on In Vitro and Ex Vivo Angiogenesis

(A) Representative images and quantification of scratch-wound healing after overexpression of a WT-microRNA mimic or ED-microRNA mimic in HUAFs relative to a non-targeting microRNA mimic (Ctrl microRNA). White lines indicate original scratch wound area. (B) Representative images and quantification of HUVEC tube formation after similar treatment with microRNA mimics as indicated. (A and B) Data are presented as mean ± SEM from three independent experiments performed with pooled cells from a total of 13 different umbilical cords. ∗p < 0.05, ∗∗p < 0.01, by a one-sample t test versus Ctrl microRNA or a two-sided Student t test to compare WT-microRNA versus ED-microRNA treatments. (C) Representative images and quantification of neovessel sprouting from aortic ring segments treated with microRNA mimics as indicated. Data are presented as mean ± SEM of at least 30 aortic segments per treatment, originating from 11 different mice. ∗p < 0.05, versus control condition unless otherwise indicated by a two-sided Student’s t test. Scale bars, 200 μm.