Figure 3.

linc00174 Regulated the Expression of VEGFA via EZH2

(A) Results of a RIP assay verified that the EZH2-binding RNA can be amplified by linc00174 primers. (B) The bioinformatics prediction presented the binding site between linc00174 and EZH2. (C) Western blot analysis was performed to detect the level of H3K27me3. (D) qRT-PCR assay was performed to determine the expression of linc00174, and the results indicated that overexpression of linc00174 stimulated the mRNA level of VEGFA. (E) Western blot analysis was performed to detect the level of VEGFA. (F) The concentration of VEGFA was detected using an ELISA assay. The data are presented as the mean ± SD obtained from at least three independent experiments. Significance was determined by a Student’s t test. ∗∗p < 0.01, ∗∗∗p < 0.001.