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. 2020 Jul 10;21:824–836. doi: 10.1016/j.omtn.2020.07.010

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

EZH2 Regulated the Expression of ZNF24 and Runx1

(A) Bioinformatics predictions indicated that EZH2 binds to the promoter of ZNF24 and Runx1. (B) A ChIP assay certified that H3K27me3 binds to the promoter of ZNF24 and Runx1. (C) An MTT assay was performed and the data showed that EZH2 knockdown resulted in the suppression of cell proliferation in HUVECs. (D) Transwell assay of cell migration indicated that EZH2 knockdown caused the inhibition of HUVEC migration. (E) EZH2 knockdown caused the inhibition of tube formation in HUVECs. (F and G) qRT-PCR (F) and western blot analysis (G) were performed, and the data revealed that EZH2 knockdown increased the mRNA and protein levels of ZNF24 and Runx1, but reduced VEGFA expression. (H) An ELISA assay was subjected to assess the level of VEGFA, and the results showed that EZH2 knockdown caused a decreasing level of secreted VEGFA. The data are presented as the mean ± SD obtained from at least three independent experiments. Significance was determined by a Student’s t test. ∗p < 0.05, ∗∗p < 0.01.