Figure 5.

VEGFA Expression Was Regulated by ZNF24 and Runx1

(A) Results of a ChIP assay indicated that ZNF24 and Runx1 are binding to the promoter of VEGFA. (B) Results of a luciferase reporter assay indicated that ZNF24 and Runx1 promoted the wild-type form of VEGFA promoter-mediated luciferase activity, but there was no significant effect for the mutant one. (C) qRT-PCR results indicated significant reduced of ZNF24 and Runx1 mRNA level in the respective gene knockdown HUVECs. (D and E) qRT-PCR (D) and western blot analysis (E) were performed, and the data revealed that knockdown of ZNF24 or Runx1 upregulated the mRNA and protein levels of VEGFA in HUVECs. The data are presented as the mean ± SD obtained from at least three independent experiments. Significance was determined by a Student’s t test. ∗p < 0.05, ∗∗p < 0.01.