Figure 6.

linc00174/EZH2 Regulated VEGFA Expression through ZNF24 and Runx1 in the Heat-Denatured Cell Model

(A and B) The expression levels of Runx1, ZNF24, VEGFA, and H3K27me3 were detected using qRT-PCR (A) and western blot analysis (B). (C and D) Effects of the EZH2 inhibitor GSK343 in the heat-denatured cell model were determined by qRT-PCR (C) and western blot analysis (D). (E) The concentration of VEGFA in the supernatant was detected by ELISA. The data are presented as the mean ± SD obtained from at least three independent experiments. Significance was determined by a Student’s t test. ∗p < 0.05, ∗∗p < 0.01.