Figure 2.

miR-324-5p Promotes Cell Proliferation and Inhibits Cell Apoptosis

(A and B) PaCa-2 and BxPC-3 cells were transfected with a control miRNA (NC) or a miR-324-5p mimic (miR). Cell proliferation and DNA replication were tested by using a CCK-8 absorbance assay (A) and EdU incorporation assay (B), respectively. (C) Cells described in (A) and (B) were subjected to flow cytometry to evaluate the cell apoptosis. Data in (A)–(C) are shown as the means ± SD. Significance was analyzed by using a Student’s t test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). (D) Protein levels of PCNA and Bax as described in (A)–(C) were determined by using western blot analysis. β-Actin was used as a loading control. Data are representative of three independent experiments.