Figure 3.

KLF3 Represses Cell Proliferation

(A and B) PaCa-2 and BxPC-3 cells were transfected with a vector expressing KLF3 or a control vector. Cell growth and DNA replication were estimated by using a CCK-8 absorbance assay (A) and EdU incorporation assay (B), respectively. Data are the means ± SD from three independent experiments. Significance was analyzed by using a Student’s t test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). (C) Cells described in (A) and (B) were subjected to flow cytometry to test the percent of cell apoptosis. Data are the means ± SD from three independent experiments. Significance was analyzed by using a Student’s t test (∗∗p < 0.01). (D and E) The protein levels of PCNA and Bax in cells described in (A) and (B), respectively, were determined by using western blot analysis. β-Actin was used as a loading control. The density of the blots from three independent experiments is shown as the mean ± SD. Significance was analyzed by using a Student’s t test (∗p < 0.05).