Figure 1.

Structures and Chemical Modification Patterns of Self-Delivering siRNA Compounds Used in the Study

(A) Panel of hsiRNAs used in this study divided into four categories based on phosphorothioate (PS) content and position. Chemical modifications include alternating 2′-O-methyl/2′-fluoro, PS backbone, and cholesterol covalently linked at the 3′ end of the sense strand by a tetraethylene glycol (TEG) linker. (B) Top row: COS-7 cells transiently expressing GFP-EEA1 were treated with 0.25 μM hsiRNA and fixed after 3 h. Middle and bottom rows: HeLa cells treated with 0.25 μM hsiRNA and immunostained with either calnexin (middle row) or LAMP1 (bottom row) and fixed after 24 h. All cells were imaged on a confocal microscope or structured illumination microscope (SIM). Scale bars, 10 μm or 1 μm for insets.