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. 2020 Aug 2;36:101669. doi: 10.1016/j.redox.2020.101669

Fig. 1.

Fig. 1

Oxidation of HDAC4 upon Nox4 overexpression and treatment with H2O2. (A) BIAM switch redox assay (n = 5) (One-way ANOVA with Tukey post-hoc test, *p < 0.05), (B) relative mRNA expression analyzed by qPCR (n > 4) and (C) relative protein expression of HDAC4 analyzed by Western blot and normalized to β-actin (n = 3) in Nox4 tet-on cells with or without tetracycline (1 μg/ml, 24 h) and DPI (3 μM, 3 h). (D) BIAM switch redox assay (n > 3) (One-way ANOVA with Dunnett post-hoc test, *p < 0.05) and (E) relative protein expression of HDAC4 in HUVECs with H2O2 (10/100/1000 μM, 30 min or 6 h) (n = 4). Representative Western blots are depicted. All bar graphs show means ± SEM.