DY-635 Uptake Depends on the Presence of OATP1B3
(A) Quantitative real-time PCR of mRNA expression of OCT1 and OATP1B3 genes after siRNA knockdown in K562 cells. (B and C) Uptake of 100 nM DY-630 (B) and DY-635 (C) from both non-transfected K562 cells (NTCs) and K562 cells immediately before and 24, 48, and 72 h after transfection with OCT1, OATP1B3, or scrambled siRNA. KD, knockdown. RFU, relative fluorescence units. ∗p < 0.05.