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. 2020 Jul 25;18:372–381. doi: 10.1016/j.omto.2020.07.007

Figure 5.

Figure 5

Functionalization of PLGA Nanoparticles with DY-635 Increases Nanoparticle Uptake in CML Cells with Stem Cell Signature

(A) SEM of DY-635 functionalized PLGA nanoparticles DY-635[NP](NileRed). Scale bar, 2 μm. (B) Schematic illustration of non-functionalized nanoparticles [NP](NileRed) and DY-635[NP](NileRed) both labled with a Nile Red core. (C) Flow cytometric results of nanoparticle incubation of K562 cells with competitive antagonism of cyclosporin A. (D) SEM of [NP](NileRed). Scale bar, 2 μm. (E) Nanoparticle uptake of NTCs and 72 h after OCT1, OATP1B3, and scrambled siRNA transfection, respectively. Shown are mean values with their standard errors. KD, knockdown. (F) Flow cytometric results of nanoparticle incubation of CML cells with stem cell signature (CD34+/CD38−/CD26+) and hematopoietic stem cells (CD34+/CD38−/CD26−). N = 5. ∗p < 0.05. ∗∗p < 0.01.