Figure 5.

Hippocampal Adult Neural Progenitor Cell Number Is Not Impaired by 3 i.c.v. ASO Injections into Atxn1^154Q/2Q Mice

(A) Western blot analysis of ATXN1 expression in the hippocampus of untreated Atxn1^2Q/2Q and Atxn1^154Q/2Q mice given either no treatment or Atxn1-targeting ASO353. (B) Representative confocal images of sections of the dentate gyrus showing NPCs as defined by immunostaining of DCX from WT untreated, Atxn1^154Q/2Q untreated, and Atxn1^154Q/2Q mice given 3 i.c.v. injections of Atxn1-targeting ASO353. (C) NPCs (DCX⁺ cells) from untreated WT mice (4 sections from animal 1 [solid symbols] and 5 sections from animal 2 [open symbols]), untreated Atxn1^154Q/2Q mice (5 sections from animal 1 [open symbols] and 4 sections from animal 2 [solid symbols]) and Atxn1^154Q/2Q mice given 3 i.c.v. injections of Atxn1-targeting ASO353 (6 sections from animal 1 [solid symbols] and 4 sections from animal 2 [open symbols]).