Fig. 1.

Regulation of iron and associated genes under hypoxia. A. Primary human macrophages were incubated under hypoxia (1% O₂, 16 h). Cellular iron was determined by atomic absorption spectroscopy. Data were normalized to the normoxic control (n = 6–7). B. Primary human macrophages were incubated for 16 h under hypoxia (1% O₂) and iron in the cell supernatants was analyzed by atomic absorption spectroscopy (n = 6–7). Data were normalized to the normoxic control. C-L. Human macrophages were incubated for indicated times under hypoxia (1% O₂), RNA of divalent metal transporter 1 (DMT1), transferrin receptor (TfR), ceruloplasmin (CP), ferroportin (FPN), ferritin heavy chain (FTH), ferritin light chain (FTL), mitochondrial ferritin (FTMT), poly(rC)-binding protein 1 (PCBP1), ubiquitin ligase E3 ubiquitin-protein ligase HERC2 (HERC2), and nuclear receptor coactivator 4 (NCOA4) was analyzed by qPCR, and normalized to TATA box binding protein (TBP). Date were normalized to the normoxic control (n = 7–8). Data are mean values with SEM. Students t-test values p < 0.05 were considered as significant.