Fig. 2.

Protein expression of ferritinophagy related proteins. A-D. Primary human macrophages were incubated for indicated times under hypoxia (1% O₂), total protein was stained, and Western analysis was performed for nuclear receptor coactivator 4 (NCOA4), ferritin heavy chain (FTH), ferritin light chain (FTL), and mitochondrial ferritin (FTMT). For quantification, the lane normalization factor (LNF) was calculated and the normoxic control was set to 1 (n = 3–4). E. Macrophages were transfected with a siRNA against NCOA4 (siNCOA4) or an untargeted control (NTC). Total protein was stained and Western analysis for FTMT, FTH, and FTL was performed. For quantification, the intensity was normalized to LNF and NTC was set to 1 (n = 4–9). Complete total protein stains are collectively shown in Fig. S1. All data are depicted with SEM. Students t-test values p < 0.05 were considered as significant.