Figure 5.

Lentiviral Integration Profile in Lymph Node Versus Liver Hepatocytes

(A) Relative integration frequency in genomic elements (TU [transcription units]) for transplanted hepatocytes that remained in lymph nodes or migrated to the liver. (B) Relative integration frequency in CpG-rich promoter regions versus other areas of the genome for transplanted hepatocytes that remained in lymph nodes or migrated to the liver. (C) Relative integration frequency based on distance upstream and downstream of transcription start sites for transplanted hepatocytes that remained in lymph nodes or migrated to the liver. (D) Relative integration frequency in tumor-associated genes versus other areas of the genome for transplanted hepatocytes that remained in lymph nodes or migrated to the liver. (E) Lenti integration into the Fah locus. 99% of the clones found in the liver were clones of cells that were present in lymph nodes. (F) Scatter dot plot graphs showing reads per kilobase million (RPKM) values for liver-enriched lenti-disrupted genes and the reporter gene (NIS) in control liver, native liver, hepatized lymph node, and control lymph node. Data are mean ± SEM.