Figure 4. .

The reciprocal expression of DENV1-NS1 and HCV-NS5A after Con1 cells successfully infected by DENV serotype 1. Con1 cells were infected with either 10 MOI of DENV or inactive DENV1 as a negative control. A, One representative result of Western blots, which were detected with 3 specific antibodies, 1 binding up to the DENV NS1 protein, another to HCV NS5A, and the other to GAPDH. B, The bar graphs of protein expression of DENV1 NS1 and HCV NS5A. The protein levels were calculated by the quantification of bands of intensity of NS1 and NS5A after normalization of GAPDH. The data are demonstrated by the mean values of triplicate assays ± SD. *P = .019 (by independent t test); ^**P = .001 (by 1-way analysis of variance trend test). Abbreviations: DENV1, dengue virus serotype 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HCV, hepatitis C virus; NS1, nonstructural protein 1; NS5A, nonstructural protein 5A.