Skip to main content
. 2020 Aug 21;6(8):e04715. doi: 10.1016/j.heliyon.2020.e04715

Figure 2.

Figure 2

Purification of bacteriocin from the CFS of Lactobacillus plantarum B21. L. plantarum A6 was used as indicator strain in all WDA assays. (a) L. plantarum B21 CFS bacteriocin activity using WDA assay before (left) 800 AU/mL and after (right) at 12,800 AU/mL concentration through an ultrafiltration cell with 10 kDa membrane cut off. (b) The WDA assay examining the antimicrobial activity of organic (left) and aqueous phase (right) from the n-butanol extraction. Well 1–4 represent four technical replicates for each assay. (c) FPLC of the active fractions from the NAP10 gel filtration column. Eluted protein was detected at 280 nm (blue line) and 214 nm (red line). Fractions F14, F15 and E15 showed the strongest antimicrobial activities based on the WDA assay.