Astrocyte Markers in the 3D Cortical Culture
(A) Relative mRNA expressions at 1, 14, and 21 DIV of Slc1a3, excitatory amino acid transporter 1, marker of astrocytes. n = 7–11 samples from three to four independent experiments ANOVA with post-hoc Tukey test was used for statistical significance. ∗∗p < 0.001. Scale bar, 100 μm.
(B) Confocal image showing the presence of astrocytes identified by the expression of glial fibrillary acidic protein (GFAP). n = 3 samples from three independent experiments.