Figure 3.

The application of human HCC cell lines with metastatic potential. A: Oct 4/Nanog induced Snail expression was significantly inhibited by a specific Stat 3 inhibitor S3I-201; B: Knockdown of Stat3 reversed Oct-4/Nanog-induced overexpression of Snail in 97 L-ON cells, indicating that co-expression of genes Oct4 and Nanog might modulate metastatic potential by Stat 3/Snail signaling pathway. Picture A and B were cited from Yin et al⁷⁹ C and D: MHCC97 L cells (C) underwent dramatic morphologic changes into mesenchymal, fibroblast-like phenotype (D) after treatment with ectopic Oct4 and Nanog expressions. Picture C and D were cited from Yin et al⁷⁹ E and F: Comparing to the control group (E), the whole-body imaging showed that the quantum dots fluorescence was localized in the pulmonary metastasis of the model (F), showing the promising function of quantum dots for predicting HCC metastasis. Picture E and F were cited from Chen et al⁸⁵ G: Other regulators and biomarkers.