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. 2020 Aug 1;23(8):101427. doi: 10.1016/j.isci.2020.101427

Figure 3.

Figure 3

Siglec-1 Mediates HIV Capture and Uropodial Compartmentalization in Motile DCs

(A) Micrograph of mature DCs pulsed with either wild-type or ΔEnv HIV-iGFP, washed, and embedded in collagen for live-cell imaging. Time stamp: min:sec.

(B) Expression of cell surface Siglec-1 on mature DCs.

(C) DCs were treated with either isotype or anti-Siglec-1 antibody prior to exposure to wild-type or ΔEnvHIV-iGFP. Numbers indicate % of DCs that captured HIV particles.

(D) Percent HIV capture by DCs. Data was normalized to isotype antibody-treated cells. Mean ± SEM are shown. Representative data from four independent experiments are shown. n.s., not significant; ∗∗∗p < 0.001. Unpaired Student's t test.

(E) Micrograph of HIV-captured DCs stained for Siglec-1 in collagen.

(F) Representative line profile analysis of GFP and Siglec-1 fluorescent intensity in a polarized, HIV-captured DC shown in (E). Representative data from three independent experiments are shown.