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. 2020 Aug 26;477(16):3019–3032. doi: 10.1042/BCJ20200385

Figure 1. Purification and composition of the 80S cytosolic ribosome in Arabidopsis cell culture.

Figure 1.

(A) Number of identified Arabidopsis proteins (AGIs) found in all of three replicates following differential centrifugation steps for low, partial and high enrichment of ribosomes. (B) The number of Arabidopsis 80S r-proteins isoforms identified across the three replicates. (C) Percentage of normalised spectral abundance factors (NSAF) in each ribosome enrichment that are r-proteins. (D) The ratio of NSAF for 60S large subunit r-proteins/40S small subunit r-proteins (RPL/RPS). (E) Principal component analysis of spectral counts assigned to the 319 proteins found by the differential centrifugation process across the three replicates and three purification steps (1–9). (F) Hierarchical clustering analysis of spectral counts for the 319 proteins identified in the purification process. The relative number of normalised spectral counts is shown in green for numbers <1 and in red for numbers >1. The vertical numbers (1–6) indicate cluster number based on Pearson correlation. Cluster 6 contains 193 proteins, including 135 80S cytosolic ribosome r-proteins. The horizontal numbers (1–9) indicate replicate number as in (E).