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. 2020 Aug 19;6(8):e04602. doi: 10.1016/j.heliyon.2020.e04602

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Up-regulating effect of EaF10 active sub-fractions on the nuclear translocation of Nrf2 in CCl₄-intoxicated cells. L-02 cells were treated without or with CCl₄ (15mM), or co-treated with CCl₄ and EaF10 active sub-fractions or silymarin (100 mg/mL) for 24h. After treatment, Nrf2 level was detected into the nuclear fraction and Keap-1 level was detected into the cytosolic fractions by western blotting. Lamin-B and β-actin were used as loading control respectively for the nuclear and cytosolic fraction. Each blot represents one of three independent experiments. A and B: effect of active sub-fraction on Nuclear Nrf2 and cytosolic Keap-1 protein expression level; and densitometry analysis of blots respectively. Values are means ± SD of three independent experiments in triplicate. ^ΔP<0.05 significantly different compared to control group. ∗P˂0.05 significantly different compared to CCl₄-intoxicated group. M: molecular weight marker; Con: control group; Sil: silymarin; EaF10: methylene chloride/methanol (90:10, v/v) fraction of E. africana; EaF10sf1: sub-fraction 1 of EaF10; EaF10sf2: sub-fraction 2 of EaF10.