Table 2.
Primer and probe sequences used in PCR assays.
| Parasite | Sequence | Partial gene | Base pair length | Reference |
|---|---|---|---|---|
| A. vasorum | 121 | a | ||
| Primer | ||||
| I2F10 (forward) | 5′-CGCATGATGAAAGAATGCTG-3′ | ITS-2 | ||
| I2R9 (reverse) | 5′-GACGACGACGACAACCACT-3′ | ITS-2 | ||
| Probe | ||||
| I2P2 | FAM-ACAACATTGCTTGTCGAACGGCGTT-BHQ1 | |||
| C. vulpis | ~90 | a | ||
| Primer | ||||
| CvITS2f (forward) | 5′-GCATGATATTCGACGATTG-3′ | ITS-2 | ||
| CvITS2r (reverse) | 5′-GTGTGATCTAGTCATGTATAAC-3′ | ITS-2 | ||
| Probe | ||||
| CvITS2p | HEX-CAGCAATGAGAAGACACTATACACAAG-BHQ1 | |||
| A. abstrusus | ~233 | b | ||
| AabFor (forward) | 5′-GTAACAACGATATTGGTACTATG-3′ | ITS-2 | ||
| AabRev (reverse) | 5′-GAACTCCTTCACGTGCTACTCG-3′ | ITS-2 | ||