Fig. 3.

Ectopic expression of GATA-4 in the benign Ben-Men-1 cell line. a RT-qPCR, densitometry analysis and representative Western blot of GATA4 and BCL2 expression levels following lentiviral-mediated GATA4 transduction (p-Lenti-GIII-GATA4). b RT-qPCR analysis of the miR-497~195 cluster expression following GATA4 overexpression, compared to p-Lenti-GIII-GFP-infected cells. c RT-qPCR, densitometry analysis and representative Western blot showing increased Cyclin D1 expression levels and Rb phosphorylation following GATA-4 overexpression, compared to p-Lenti-GIII-GFP-infected cells. d Cell viability measured by ATP assay following p-Lenti-GIII-GATA4 transduction compared to control cells. Data are reported as mean ± SEM (Student's t-Test; NS = not significant; * = p<0•05, ** = p<0•01, *** = p<0•005). Transduction has been performed in three independent experiments, with one replica per repeat, to ensure data consistency.