Fig. 4.

Lentiviral-mediated GATA4 RNA interference in KT21-MG1 cells. a RT-qPCR, densitometry analysis and representative Western blot showing levels of GATA4 and BCL2 following shGATA4 infection, compared to scramble-infected cells. b RT-qPCR analysis of the miR-497~195 cluster expression following shGATA4 infection. c RT-qPCR, densitometry analysis and representative Western blot showing Cyclin D1 and Rb phosphorylation levels following GATA4 knockdown, compared to scramble. d Cell viability measured by ATP assay following shGATA4 transduction compared to scramble-infected cells. Data are reported as mean ± SEM (Student's t-Test; NS = not significant; * = p<0•05, ** = p<0•01, *** = p<0•005). Transduction has been performed in three independent experiments, with one replica per repeat, to ensure data consistency.