Subject	anaesthesiology and pain medicine
Specific subject area	Glucocorticoids
Type of data	Graphs (within manuscript) Figures (within manuscript) Table (within repository file)
How data were acquired	Flowcytometry of peripheral blood mononuclear cells from healthy volunteers was performed to obtain data. Stained samples were acquired on a 4-laser LSR Fortessa (BD Biosciences, San Jose, CA, USA) with BD FACSDiva software (BD Biosciences, NJ, USA). The analysis of acquired samples was performed using FlowJo data analysis software (FlowJo, LLC, Ashland, OR, USA). All statistical analyses were performed within Prism 7, Graphpad software (La Jolla, CA, USA).
Data format	Raw (Mendeley data repository “Data on the early in-vivo effects of a single anti-emetic dose of dexamethasone on the surface marker expression of various leucocyte subsets”, Mendeley Data, V1, doi: 10.17632/hv6v26bczp.1), and Analysed (within this manuscript)
Parameters for data collection	10 healthy male volunteers aged between 20 and 35 years. Baseline blood was collected after a 22-g intravenous (i.v.) cannula was sited. Dexamethasone (8 mg) was then injected and blood collected again at 2 h, 4 h and 24 h. The baseline and 24-h bloods were collected between 06:45 h and 1100 h. PBMCs were then isolated and analysed using flowcytometry.
Description of data collection	Surface marker expression strength on PBMCs isolated from whole blood described as mean fluorescence intensity (MFI) Cell activation/maturation: CD83, CD86, HLA-DR Cell migration: CCR4, CCR7 Inhibitory signalling; PD-L1 Programmed cell death: PD-1, CD95 Responsiveness to signalling: TNFR2, CTLA4 TGF-b release: LAP Cell viability: Zombie yellow stain for intracellular amines
Data source location	Institution: Australian Centre for Blood Diseases City/Town/Region: Melbourne, Victoria Country: Australia
Data accessibility	Data incorporated within this article and the Mendeley data repository “Data on the early in-vivo effects of a single anti-emetic dose of dexamethasone on the surface marker expression of various leucocyte subsets”, Mendeley Data, V1, doi: 10.17632/hv6v26bczp.1
Related research article	C.R. Bain et al.; The early in-vivo effects of a single anti-emetic dose of dexamethasone on innate immune cell gene expression and activation in healthy volunteers; Anaesthesia; doi 10.1111/anae.14306