Fig. 1.

CGRP and CGRP receptor are expressed in the fracture callus on mRNA level. (a) Relative serum CGRP levels in WT and αCGRP-deficient mice with a femoral osteotomy stabilized with an external fixator 14 days post-injury. n = 4–6 mice per group (unpaired student's t-test). (b, c) Gene expression (virtual copy numbers per Gapdh) of the indicated genes in the intact femoral shaft of control animals (intact) and in the fracture callus at the respective time points during bone regeneration (d3, d7, d14). Calca = encoding αCGRP; Crlr = calcitonin receptor-like receptor; Ramp1 = receptor activity-modifying protein 1. n = 4–6 mice per group (one-way Anova followed by Tukey post-hoc test). (d) Calca expression (fold) in the indicated tissues 3 and 7 days following Fx. Hypoth. = hypothalamus; WAT = white adipose tissue; BAT = brown adipose tissue; n.d. = not detectable. The dotted red line indicates average expression of respective, untreated control tissues. n = 3–5 mice per group (unpaired student's t-test). For (a-d), box plots represent median with minimum and maximum whiskers. Controls are untreated mice of the same sex and age.