Fig. 4.

Impaired callus formation in αCGRP-deficient mice. (a) Representative callus sections (Movat Pentachrome staining) of WT and αCGRP-deficient mice at the indicated time points (yellow = mineralized bone; green = cartilage; red = muscle). Black dotted line indicates fractured bone cortices within the developing callus. (b, c) Histomorphometric quantification of static callus parameters in the same mice. Scale bars = 200 μm. n = 4–6 as indicated per group and time point. Box plots represent median with minimum and maximum whiskers. For (b) and (c), the different timepoints were evaluated independently and compared by unpaired student's t-test.