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. 2020 Aug 24;59:102970. doi: 10.1016/j.ebiom.2020.102970

Fig. 6.

Fig. 6

αCGRP-deficient mice display profound alterations in callus bone cell distribution. (a) Representative callus images demonstrating osteocalcin-positive (brown), bone-forming osteoblasts (black arrows) in WT and αCGRP-deficient mice at d21 following surgery. Scale bars = 100 μm. (b) Histomorphometric quantification of osteoblast parameters in the callus of the same mice at the indicated time points. tObN/ROI = total osteoblast numbers in the callus area; ObN/Bpm = osteoblast numbers per bone perimeter; ObS/BS = osteoblast surface per bone surface. (c) Representative callus images (TRAP activity staining) demonstrating tissue-resorbing osteoclasts (red staining, arrows) in WT and αCGRP-deficient mice at the indicated time points. Scale bars = 200 μm. (d) Histomorphometric quantification of osteoclast parameters in the callus of the same mice. tOcN/ROI = total osteoclast numbers in the callus area; OcN/Bpm = osteoclast numbers per bone perimeter; OcS/BS = osteoclast surface per bone surface. For (b) and (d), n = 4–6 as indicated per group and time point. The different timepoints were evaluated independently and compared by unpaired student's t-test. Box plots represent median with minimum and maximum whiskers.