Fig. 7.

Osteogenesis and osteoclastogenesis is not affected in bone-marrow cells derived from αCGRP-deficient mice. (a) TRAP activity staining of WT and αCGRP-deficient, bone marrow-derived osteoclasts differentiated in the presence of M-CSF and RANKL (staining performed at day 6 of differentiation). Scale bars = 50 μm. The quantification of osteoclast numbers per viewing field is depicted on the right (Ocl.N./VF). n = 6 independent cultures per group. (b) Alizarin red staining of bone marrow-derived osteoblasts from the same mice differentiated in the presence of ascorbic acid and β-glycerophosphate at day 10 of osteogenic differentiation. Scale bar = 4 mm. The quantification of extracellular matrix mineralization is depicted on the right. n = 8 independent cultures per group. (c) qRT-PCR expression analysis (fold) for the indicated genes in bone marrow-derived osteoblasts at day 2 of osteogenic differentiation with ascorbic acid and β-glycerophosphate, stimulated with CGRP (10⁻⁷ M) for 6 h. n = 4 independent cultures per group (unpaired student's t-test).