Fig. 8.
Decreased expression of genes associated with bone formation, remodeling, and PPARγ signaling in the callus of αCGRP-deficient mice. (a) Average (Avg log2) and relative (fold) expression of genes with significant reduction in day 7 callus tissue from 12-week-old female mice using genome-wide expression analysis (αCGRP-deficient mice vs. WT mice; n = 3 per genotype). (b) qRT-PCR expression analysis (virtual copy numbers per Gapdh) for the indicated genes in bone marrow-derived osteoblasts at day 2 of osteogenic differentiation with ascorbic acid and β-glycerophosphate, stimulated with αCGRP (10−7 M) and olcegepant (1μg/ml; BIBN) for 6 h as indicated. n = 4–6 independent cultures per group (two-way Anova followed by Tukey post-hoc test).