Table 2. Summary of equilibrium and kinetic measurements of Puf4 affinity.
| Equilibrium* | Kinetic | ||||
|---|---|---|---|---|---|
| Temperature,°C | KD(hyperbolic), pM | KD(quadratic), pM | kon, M−1s−1* | koff, s−1 | KD (=koff/kon), pM |
| 0 | ≤1.7 | 1.39 ± 0.09 | (2.85 ± 0.14)×107 | (2.92 ± 0.17)×10−5 | 1.02 ± 0.08 |
| 25 | 120 ± 30 | 120 ± 30 | (1.04 ± 0.14)×108 | 0.014 ± 0.003 | 130 ± 30 |
*The values have been normalized by active protein fraction (75–90%). KD(hyperbolic) and KD(quadratic) refer to values derived from fits to Equation 4b and Equation 5, respectively. Errors are defined in Materials and methods.