Figure 1.

In vitro electrical stimulation increases the number of neurospheres. Ai, Aii, Experimental paradigms for in vitro electrical stimulation. B, C, Fold change in the number of neurospheres from stim-off cells and stim-on cells from (B) primary cultures (n = 3 independent experiments, *p = 0.03) and (C) neurosphere-derived NPCs (n = 4 independent experiments, ****p < 0.0001). Fold change in the number of neurospheres cultured using CM from stim-off and stim-on (D) primary cells (n = 3 independent experiments) and (E) neurosphere-derived NPCs (p = 0.5 and p = 0.9, respectively; n = 3 independent experiments) Each point in the graph represents an independent experiment, plotted with mean ± SEM. Analysis performed via a two-tailed unpaired t test between stim-off and stim-on groups. Neurosphere counts from in vitro stimulation found in Extended Data Figure 1-1. Comparison of fold changes in neurospheres following stimulation with and without Wnt inhibitor to inhibit symmetric divisions found in Extended Data Figure 1-2.