TABLE 2.
Proportion of high‐molecular‐weight DNA (20 kbp) over time when samples are pooled per solution treatment
Treatment | High‐molecular‐weight DNA % | High‐molecular‐weight DNA % (standardized) | Scale factor between DESS and ETOH | ||
---|---|---|---|---|---|
DESS | ETOH | DESS | ETOH | DESS/ETOH | |
1 day | 15.8 | 11.4 | 100.0 | 13.3 | 7.5 |
1 week | 14.1 | 9.6 | 85.6 | 10.8 | 7.9 |
2 weeks | 11.1 | 6.6 | 62.2 | 6.9 | 9.0 |
1 month | 7.0 | 9.1 | 37.9 | 12.2 | 3.1 |
3 months | 15.1 | 11.9 | 57.3 | 21.9 | 2.6 |
DESS: solution of 20% DMSO, 0.25 M EDTA, and NaCl saturated. ETOH: 99.5% ethanol. The standardized percentages of high‐molecular‐weight were obtained by taking the plot with largest contribution of high‐molecular‐weight (i.e., largest area under the curve above 20 kbp, DESS—1 day) and using that to standardize all other high‐molecular‐weight contributions.