Figure 5.

NR treatment preserves nuclei of the outer nuclear layer in the LIRD mice. (A–C) Representative H&E images of retina sections from each group at the region of 250 to 750 µm from the optic nerve head. Complete sections are shown in Supplementary Figure S1. (D). One week after LIRD induction, nuclei were counted in eight discrete regions of retinal sections starting at 250 µm from the optic nerve head and extending every 500 µm outward along both the dorsal/superior (positive values on abscissa) and ventral periphery/inferior (negative numbers on abscissa). PBS, bright–treated mice (red) showed significant loss of nuclei at six distances from the optic nerve head compared with the control group (black). However, NR treated mice (green), exhibited mean nuclei counts statistically indistinguishable from that of the control group (black) throughout the length of the retina. ***P < 0.001, ****P < 0.0001 versus other groups by two-way ANOVA with Newman-Keuls multiple comparisons post hoc test. N = 3–6 mice per group. Error bars represent SEM. Size marker = 50 µm.