Table 1.
Sequences of the primers used in cloning, mutagenesis, RNA interference, qPCR analyses, ChIP studies, and luciferase reporter experiments
| Gene | Use | Primer ID | Sequence (5′ → 3′) |
|---|---|---|---|
| ACTB | qPCR | b-ActinFw | TGTGGCATCCACGAAACTAC |
| qPCR | b-ActinRv | TCATACTCCTGCTTGCTGATCC | |
| ANGPTL4 | ChIP-qPCR | ANGPTL4_cQ_F | AAGTGTATGAGTGGCAGCCT |
| ChIP-qPCR | ANGPTL4_cQ_R | AACTTGCACCGATCTCCTCT | |
| Cloning | ANGPTL4_B_F | GCGAGATCTCACGGTTCGTAGAGGAAGGC | |
| Cloning | ANGPTL4_B_R | GCGAAGCTTCCCACTCCTGTCCATACCCT | |
| EIF5A | ChIP-qPCR | EIF5A_cQ_F | TGGAGATGGGTAGGGTGTGT |
| ChIP-qPCR | EIF5A_cQ_R | GACCAACCAAGCAGCCCTAT | |
| EPAS1 | qPCR | Q_HIF2a_F | CCCAGATCCACCATTACAT |
| qPCR | Q_HIF2a_R | ACTCCAGCTGTCGCTTCA | |
| EPO | qPCR | EPO_RT_F | CTCCGAACAATCACTGCT |
| qPCR | EPO_RT_R | GGTCATCTGTCCCCTGTCCT | |
| (Control) | ChIP-qPCR | EPO_ctrl_cQ_F | GGAAGGCAATTTTGTGTGCG |
| (Control) | ChIP-qPCR | EPO_ctrl_cQ_R | CCAAGCACCAGAAACTCACC |
| ChIP-qPCR | EPO_cQ_F | CCAGTGGAGAGGAAGCTGAT | |
| ChIP-qPCR | EPO_cQ_R | CTTCCTTCATCCCCACGTCT | |
| Cloning | EPO-1_F | GCGGAGCTCGGATTGTGGGAAGGGAGACC | |
| Cloning | EPO-1_R | GCGCTCGAGATAGCCGGGGCGCTAAATC | |
| EPOR | ChIP-qPCR | EPOR_cQ_F | TAGGCAGCGAACACCAGAAG |
| ChIP-qPCR | EPOR_cQ_R | TCACACACACACACAAGGCT | |
| HIF1A | qPCR | Q_HIF1a_F | CTAGCTTTGCAGAATGCTCAG |
| qPCR | Q_HIF1a_R | GTAGTAGCTGCATGATCGTCTG | |
| HIF3A | RNAi | siHIF3A_a | UAACAGGGCAGUAUCGCUU |
| RNAi | siHIF3A_b | CGACAGGAUUGCAGAAGUG | |
| RNAi | siHIF3A_c | GCAAGAGCAUCCACACCUU | |
| RNAi | siHIF3A_d | GAACUGCUCUGGACAUAUG | |
| RNAi | siHIF3A_e | SR312024B, sequence not available | |
| qPCR | Q_HIF3a_all_F | CCCCACGGAGCGGTGCTTCT | |
| qPCR | Q_HIF3a_all_R | AGTCTGCGCAGGTGGCTTGT | |
| HIST1H2BK | qPCR | HIST1H2BK_Q_F | TGCTGCTCGTCTCAGGCTCGT |
| qPCR | HIST1H2BK_Q_R | CTCTCCTTGCGGCTGCGCTT | |
| ChIP-qPCR | HIST1H2BK_cQ_F | GGGCCCCTAAGCTTTCAACA | |
| ChIP-qPCR | HIST1H2BK_cQ_R | GGCTCTTCTGGCCTTGGAAA | |
| Cloning | HIST1H2BK_R | GCGGAGCTCCGGCGTCGAGTTAATCTTGT | |
| Cloning | HIST1H2BK_R | GCGCTCGAGTCCGGTTTTCAGTCTGGTCC | |
| Mutagenesis | HIST1H2BK_HRE1_F | AAGACGGTCACCGCCATGGTAAATGTCTACGCGCTCAAGCGCC | |
| Mutagenesis | HIST1H2BK_HRE1_R | GGCGCTTGAGCGCGTAGACATTTACCATGGCGGTGACCGTCTT | |
| Mutagenesis | HIST1H2BK_HRE2_F | GCCGTGACCTACACGGAGTAAATCAAGCGCAAGACGGTCAC | |
| Mutagenesis | HIST1H2BK_HRE2_R | GTGACCGTCTTGCGCTTGATTTACTCCGTGTAGGTCACGGC | |
| Mutagenesis | HIST1H2BK_HRE3_F | TGTTGAAGGTGTTCCTGGAGATAAATATCCGGGACGCCGTGACCTACAC | |
| Mutagenesis | HIST1H2BK_HRE3_R | GTGTAGGTCACGGCGTCCCGGATATTTATCTCCAGGAACACCTTCAACA | |
| Mutagenesis | HIST1H2BK_HRE4_F | TGCTCGCCGCGGCGTAAATAAGCGCATTTCTGGCCTCATCTATGAG | |
| Mutagenesis | HIST1H2BK_HRE4_R | CTCATAGATGAGGCCAGAAATGCGCTTATTTACGCCGCGGCGAGCA | |
| HPRT1 | qPCR | hHprt_F | CCTGGCGTCGTGATTAGTGAT |
| qPCR | hHprt_R | AGACGTTCAGTCCTGTCCATAA | |
| PMB6 | qPCR | hPMB6qfor | AGCGACACCACAAAGAGTTCA |
| qPCR | hPMB6qrev | GCTGATGCTCCTGTAAGACTTGA | |
| PSMD5 | ChIP-qPCR | PSMD5_cQ_F | AATCTTGATCCTGGGCCAGC |
| ChIP-qPCR | PSMD5_cQ_R | GCGCACGTCCCTATTACTCA | |
| PTX3 | qPCR | hPTXqfor | CATCTCCTTGCGATTCTGTTTTG |
| qPCR | hPTXqrev | CCATTCCGAGTGCTCCTGA | |
| SLC6A14 | qPCR | hSLC6A14qfor | ACCGTGGTAACTGGTCCAAAA |
| qPCR | hSLC6A14qrev | CGCCTCCACCATTGCTGTAG | |
| TBP | qPCR | TBP_Q_F | GAATATAATCCCAAGCGGTTTG |
| qPCR | TBP_Q_R | ACTTCACATCACAGCTCCCC | |
| TMEM27 | qPCR | hTMEM27qfor | CTGGTGACTGCCATTCATGCT |
| qPCR | hTMEM27qrev | CCATCGCTTTGAAGAGGTATTCT |