Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 27;11:4285. doi: 10.1038/s41467-020-17949-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a, b Monitoring of fluorescently labelled cytokinin iP-NBD (green) and ER-marker p24δ5-RFP (red) in LRC cells (a) and root meristematic epidermal cells (b). iP-NBD detected partially co-localizing with p24δ5-RFP in ER (red arrowheads) and in non-ER cellular structures (white arrowheads). c–f Monitoring of iP-NBD (green) and FM4-64 (red, membrane selective dye) in LRC (c, d) and root meristematic epidermal cells (e, f). White arrowheads (c, e) indicate co-localization of iP-NBD and FM4-64 in vesicles. Profiles of fluorescence intensity distribution of both FM4-64 (red line) and iP-NBD (green line) in LRC (d) and epidermal (f) cells were measured along the white lines (c, d) starting from upper end (0 µm) towards the arrowhead. Peaks of FM4-64 fluorescence maxima (black arrows) correlate with the plasma membrane staining. iP-NBD fluorescence maximum does not overlap with FM4-64 fluorescence peak at the plasma membrane in LRC cell (d). Peaks of iP-NBD signal partially overlap with FM4-64 maxima and indicate presence of cytokinin fluoroprobe at the plasma membrane of epidermal cells (f). g Co-localization of iP-NBD and FM4-64 in endosomal compartments (blue arrowheads) formed in root meristematic epidermal cells treated with 50 µM in BFA for 1 h. Scale bars = 5 μm.