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. 2020 Aug 27;11:4285. doi: 10.1038/s41467-020-17949-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–e Monitoring of CRE1/AHK4-GFP cytokinin receptor (green) and ER-marker p24δ5-RFP (red) in LRC cells (a, b) and root meristematic epidermal cells (c–e). Red arrowheads mark areas of co-localization. Fluorescence intensity profiles of the ER marker (red line) and CRE1/AHK4-GFP (green line) (b, e) were measured along the white lines (a, c) starting from upper end (0 µm) towards the arrowhead. Peaks of p24δ5-RFP fluorescence maxima at the endoplasmic reticulum overlap with CRE1/AHK4-GFP signal maxima in the LRC cells and root meristematic epidermal cells (black arrows; b, e). Peak of CRE1/AHK4-GFP non-overlapping with peaks of p24δ5-RFP in the root meristematic epidermal cells indicates localization at the plasma membrane (black arrowhead; e). Detailed view (d; white arrows point to CRE1/AHK4-GFP signal at the area of the PM). Scale bars = 5 μm.