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. 2020 Aug 27;11:4287. doi: 10.1038/s41467-020-18066-8

Fig. 5. No evidence for a redundant role of the pseudogene DDX12p in cohesion and proliferation.

Fig. 5

a RPE1-hTERT cells were transfected with siRNA to DDX11 (1 nM or 10 nM) and analyzed for DDX11 and DDX12p mRNA levels using specific qRT-PCR. The specificity of qRT-PCR primers was validated in Supplementary Fig. 5. b DDX12p mRNA levels were assessed in three SV40 transformed control fibroblasts and five WABS fibroblasts. c CRISPR design for constructing DDX11 and DDX12p knockouts in RPE1-hTERT-TP53KO cells. For more detailed information and validation of clones, see Supplementary Fig. 6. d A panel of RPE1-hTERT-TP53KO cells containing specific DDX11 and/or DDX12p knockout was seeded in 96-wells plates and growth rate was analyzed using IncuCyte software. The resulting growth curves were used to calculate doubling times. e The same panel was analyzed for cohesion defects. As control, RPE1-hTERT-TP53KO cells were transfected with siDDX11 for two days. Per condition, in total 100 metaphases from two independent experiments were assessed.