Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source |
|---|---|---|
| Bt strains | ||
| JW-1, 51-4, 68-6, 69-1, 69-3, 88-1, 99-3, 214-4 | Wild-type strains with insecticidal activity | This study |
| Bti | Purified from Bacillus thuringiensis subsp. israelensis wettable powder | Wuhan Kernel |
| Edible fungus strains | Pleurotus ostreatus, Pleurotus geesteranus, Auricularia polytricha | This study |
| Escherichia coli | ||
| TOP10 | F- mcrA Δ (mrr-hsd RMS-mcrBC) φ80 lacZΔM15 △lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galK rps (Strr) endA1 nupG | Zoonbio |
| Arctic | F–ompT hsdS(rB– mB–) dcm + Tetr gal λ(DE3) end A Hte [cpn10 cpn60 Gentr] [argU proL Strr] | |
| Plasmids | ||
| PMAL-c5x | Expression vector for cyt2, MBP, Apr | Zoonbio |
| pCYT-2 | pMAL-c5x carrying 792-bp PCR product of the cyt2, Apr | This study |